Genetic diversity among ‘Candidatus Liberibacter asiaticus’ isolates based on single nucleotide polymorphisms in 16S rRNA and ribosomal protein genes
Articolo
Data di Pubblicazione:
2009
Citazione:
Genetic diversity among ‘Candidatus Liberibacter asiaticus’ isolates based on single nucleotide polymorphisms in 16S rRNA and ribosomal protein genes / C.R. Adkar Purushothama, F. Quaglino, P. Casati, J. Gottravalli Ramanayaka , P.A. Bianco. - In: ANNALS OF MICROBIOLOGY. - ISSN 1590-4261. - 59:4(2009 Dec), pp. 681-688. [10.1007/BF03179208]
Abstract:
Citrus Huanglongbing (HLB) is one of the most destructive and widespread diseases of citrus, caused by ‘Candidatus
Liberibacter’, a non-cultured, phloem-restricted alpha-proteobacterium. In the present study, ‘Ca. Liberibacter’
was detected in symptomatic citrus plants growing in the Karnataka state (South India) by amplification of 16S rRNA and
β-operon ribosomal protein (β-rp) genes, using PCR primers specific for African and Asian ‘Ca. Liberibacter’. Sequencing
of the 16S rRNA amplified fragment revealed that ‘Ca. Liberibacter’, here identified belongs to the species ‘Ca. Liberibacter
asiaticus’. The alignments of 16S rRNA and ribosomal protein gene sequences of all known ‘Ca. Liberibacter asiaticus’-
related, allowed to recognize Single Nucleotide Polymorphisms (SNPs). Basing on SNP analysis, the strains were grouped
in fourteen 16SrRNA SNP genetic lineages (16Sr-I to 16Sr-XIV) and three β-rp SNP genetic lineages (rp-I to rp-III). Only
the strains of ‘Ca. Liberibacter asiaticus’ from the Karnataka state belonged to the genetic lineages 16Sr-I and rp-I. RFLP
diagnostic tests on the discriminative 16S rRNA SNPs were set up to identify this lineage. These results revealed the presence
of a new ‘Ca. Liberibacter asiaticus’ genetic lineage in the Indian sub-continent, where at least four genetically diverse
SNP lineages were found. These findings could open new opportunities for in-depth studies on biological niches and traits
of ‘Ca. Liberibacter asiaticus’.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
'Candidatus Liberibacter'; Genetic lineage; Huanglongbing (HLB); Single nucleotide polymorphisms
Elenco autori:
C.R. Adkar Purushothama, F. Quaglino, P. Casati, J. Gottravalli Ramanayaka, P.A. Bianco
Link alla scheda completa: