New 16Sr subgroups and distinct single nucleotide polymorphism lineages among grapevine Bois noir phytoplasma populations
Articolo
Data di Pubblicazione:
2009
Citazione:
New 16Sr subgroups and distinct single nucleotide polymorphism lineages among grapevine Bois noir phytoplasma populations / F. Quaglino, Y. Zhao, P.A. Bianco, W. Wei, P. Casati, G. Durante, R.E. Davis. - In: ANNALS OF APPLIED BIOLOGY. - ISSN 0003-4746. - 154:2(2009), pp. 279-289. [10.1111/j.1744-7348.2008.00294.x]
Abstract:
Bois noir (BN) is an insect-transmitted grapevine yellows disease caused by
phytoplasmas belonging to the stolbur subgroup 16SrXII-A. In Italy, increasing
prevalence of stolbur phytoplasma strains in vineyards suggests progressive
spread of the disease and potential for heavy impacts on the wine industry. In
this study, we investigated the genetic diversity of stolbur phytoplasma strains in
BN phytoplasma populations. Nucleotide sequences of 16S rRNA genes from
stolbur phytoplasma strains affecting vineyards in the Lombardy region of
Italy and stolbur phytoplasma 16S rDNA sequences retrieved from GenBank
were subjected to virtual restriction fragment length polymorphism analysis.
Calculation of virtual restriction similarity coefficients revealed the presence
of new subgroups in group 16SrXII (stolbur phytoplasma group). Representative
strains of confirmed new subgroups 16SrXII-F (XII-F) and XII-G and tentative
new subgroups XII-A1 through XII-A19, XII-H, XII-I, and XII-J as well
as known subgroup XII-A were from grapevines; strains representing three
additional tentative new subgroups (XII-K, XII-L and XII-M) were from other
plant hosts. Nucleotide sequence alignments identified no less than nine
genetically distinct 16S rDNA single nucleotide polymorphism lineages from
grapevine, indicating a high degree of genetic heterogeneity within BN phytoplasma
populations. The findings open new opportunities for in-depth studies
of the distribution of grapevine-associated 16SrXII phytoplasma strains in
weeds, insect vector populations and grapevines from vineyards located in different
geographic areas.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
genetic diversity ; phytoplasma classification ; restriction fragment length polymorphism ; single nucleotide polymorphisms
Elenco autori:
F. Quaglino, Y. Zhao, P.A. Bianco, W. Wei, P. Casati, G. Durante, R.E. Davis
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