Autolytic activity and pediocin-induced lysis in Pediococcus acidilactici and Pediococcus pentosaceus strains
Articolo
Data di Pubblicazione:
2003
Citazione:
Autolytic activity and pediocin-induced lysis in Pediococcus
acidilactici and Pediococcus pentosaceus strains / D. Mora, F. Musacchio, M.G. Fortina, L. Senini, P.L. Manachini. - In: JOURNAL OF APPLIED MICROBIOLOGY. - ISSN 1364-5072. - 94:4(2003), pp. 561-570.
Abstract:
Aims: To evaluate the autolytic phenotype of Pediococcus acidilactici and P. pentosaceus, the peptidoglycan
hydrolases content and the effect of pediocin AcH/PA-1 and autolysins on cell lysis.
Methods and Results: The autolytic phenotype of Pediococcus strains was evaluated under starvation conditions in
potassium phosphate buffer. The strains tested showed an extent of autolysis ranging between 40 and 90% after
48 h of starvation at 37 C. Peptidoglycan hydrolase content was evaluated by renaturing sodium dodecyl sulphate–
polyacrylamide gel electrophoresis (SDS–PAGE) using cells of Micrococcus lysodeikticus as a target for the enzymatic
activity and a major activity band migrating at about 116 kDa was detected. Additional secondary lytic bands
migrating in a range of molecular weight between 45 and 110 kDa were also detected. The lytic activity, evaluated in
the presence of different chemicals, was retained in 15 mM CaCl2 and in a range of pH between 5 and 9Æ5 but was
strongly reduced in presence of 8% NaCl and in the presence of protease inhibitors.
The substrate specificity of peptidoglycan hydrolases of Pediococcus strains was evaluated in renaturing SDS–PAGE
incorporating cells of different bacterial species. Lytic activity was detected against cells of Lactococcus lactis subsp.
lactis, L. delbrueckii subsp. bulgaricus, Lactobacillus helveticus and Listeria monocytogenes. The interaction between
pediocin AcH/PA-1 and autolysis was evaluated and a relevant effect of bacteriocin in cell-induced lysis was
observed.
Conclusions: The autolytic phenotype is widely distributed among P. acidilactici and P. pentosaceus and the rate of
autolysis is high in the majority of the analysed strains. Several autolytic bands, detected by renaturing SDS–PAGE,
retained their activity against several lactic acid bacteria and L. monocytogenes.
Significance and Impact of the Study: The characterization of the autolytic phenotype of Pediococcus acidilactici
and P. pentosaceus strains should expand the knowledge of their role in fermentation processes where these species
occur as primary or secondary bacterial population.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Autolysis; Pediocin AcH/PA-1; Pediococcus acidilactici; Pediococcus pentosaceus
Elenco autori:
D. Mora, F. Musacchio, M.G. Fortina, L. Senini, P.L. Manachini
Link alla scheda completa: