Rapid detection and quantification of plasmid-mediated colistin resistance genes (mcr-1 to mcr-5) by real-time PCR in bacterial and environmental samples
Articolo
Data di Pubblicazione:
2020
Citazione:
Rapid detection and quantification of plasmid-mediated colistin resistance genes (mcr-1 to mcr-5) by real-time PCR in bacterial and environmental samples / R. Tolosi, I. Apostolakos, A. Laconi, L. Carraro, G. Grilli, P. Cagnardi, A. Piccirillo. - In: JOURNAL OF APPLIED MICROBIOLOGY. - ISSN 1364-5072. - 179:6(2020 Dec), pp. 1523-1529. [10.1111/jam.14738]
Abstract:
Aim: The aim of the study was to validate a rapid method to detect and
quantify colistin resistance genes (mcr-1 to mcr-5) by real-time polymerase
chain reaction (RT-PCR) in diverse matrices.
Methods and results: The detection limit of two newly designed SYBR Green
real-time PCR assays for mcr-4 and mcr-5 and of previously published
protocols for mcr-1 to mcr-3 was assessed using serial dilutions of reference
strains. The assays could detect all five mcr genes with the lower limit of 102
copy numbers. Escherichia coli isolates (n = 1062) and environmental samples
(n = 93) were tested for the presence of mcr genes. The assays enabled the
detection of colistin resistance genes both in bacterial isolates and in complex
environmental samples.
Conclusions: This method represents a set of sensitive, rapid and effective
assays for the screening of colistin resistance directly from the environment.
Significance and Impact of the Study: Colistin is an antimicrobial commonly
used in animals and has recently emerged as a last-resort treatment in humans.
Plasmid-mediated mcr genes confer resistance to colistin and represent a major
threat for public health since they can be easily disseminated through
horizontal gene transfer. The rapid and sensitive detection of mcr genes is of
utmost necessity.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
E. coli (all potentially pathogenic types); antimicrobials; detection; environmental; resistance;
Elenco autori:
R. Tolosi, I. Apostolakos, A. Laconi, L. Carraro, G. Grilli, P. Cagnardi, A. Piccirillo
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