Data di Pubblicazione:
2008
Citazione:
In vitro characterization of embryionic ST14A-cells / M. Hovakimyan, K. Weinreich, S.J. Haas, E. Cattaneo, A. Rolfs, A. Wree. - In: INTERNATIONAL JOURNAL OF NEUROSCIENCE. - ISSN 0020-7454. - 118:11(2008 Nov), pp. 1489-1501.
Abstract:
The embryonic striatal temperature sensitive immortalized ST14A-cell line was characterized in vitro by immunocytochemistry when cultured at 33 degrees C and at nonpermissive temperature of 39 degrees C for up to 14 days. At 33 degrees C in DMEM/10% FCS, cells proliferated, were extensively expressing the neural progenitor cell markers nestin and vimentin contrary to neuronal markers. However, when cultured at 39 degrees C the proliferation was delayed and cells began to increase the expression of neuronal markers, followed by a decrease of nestin and vimentin. In serum-free medium the process of neuronal differentiation became more obvious, indicating the potential to use these cells for experimental restorative therapies
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
cell transplantation ; differentiation ; Huntington's disease ; immortalization ; neural progenitors ; striatum
Elenco autori:
M. Hovakimyan, K. Weinreich, S.J. Haas, E. Cattaneo, A. Rolfs, A. Wree
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