Bleaching melanin in formalin-fixed and paraffin-embedded melanoma specimens using visible light : a pilot study
Articolo
Data di Pubblicazione:
2019
Citazione:
Bleaching melanin in formalin-fixed and paraffin-embedded melanoma specimens using visible light : a pilot study / C. Pigoli, L. Gibelli, M. Caniatti, L. Moretti, G. Sironi, C. Giudice. - In: EUROPEAN JOURNAL OF HISTOCHEMISTRY. - ISSN 1121-760X. - 63:4(2019), pp. 214-221. [10.4081/ejh.2019.3071]
Abstract:
In fluorescence microscopy, light radiation can be used to bleach fluorescent
molecules in formalin-fixed and paraffin-embedded (FFPE) samples, in order to
increase the ratio between signal of interest and background autofluorescence. We
tested if the same principle can be exploited in bright field microscopy to
bleach pigmented melanoma FFPE sections together with cell morphology
maintenance. After dewaxing and rehydration, serial FFPE sections of a feline
diffuse iris melanoma, a canine dermal melanoma, a gray horse dermal melanoma and
a swine cutaneous melanoma were irradiated with visible light for 1, 2, 3, 4 and
5 days, prior to Hematoxylin and Eosin staining. Complete bleaching was obtained
after 1-day treatment in feline and swine melanomas, while 2 and 3 days were
required in canine and equine neoplasms, respectively. In all treated samples,
cell morphology was maintained. Photo-induced bleaching combined with
immunohistochemistry was tested after a 3-day photo-treatment using five
different markers. According to the literature, in all samples neoplastic cells
stained positive for vimentin, S100 and PNL2, while negative for FVIII and
pancytokeratin. In conclusion, visible light can be effectively exploited to
bleach pigmented melanoma FFPE sections prior to perform routine histochemical
and immunohistochemical stains.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Bright-field microscopy; canine; equine; feline; light radiation; photobleaching; swine
Elenco autori:
C. Pigoli, L. Gibelli, M. Caniatti, L. Moretti, G. Sironi, C. Giudice
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