Direct microfabrication of topographical and chemical cues for the guided growth of neural cell networks on polyamidoamine hydrogels
Articolo
Data di Pubblicazione:
2010
Citazione:
Direct microfabrication of topographical and chemical cues for the guided growth of neural cell networks on polyamidoamine hydrogels / LINO DOS REIS GABRIEL ARMANDO, FENILI FABIO, GIANFELICE ANTONELLA, G. Bongiorno, D. Marchesi, SCOPELLITI PASQUALE, A. Borgonovo, A. Podesta, INDRIERI MARCO, E. Ranucci, FERRUTI PAOLO, C. Lenardi, P. Milani. - In: MACROMOLECULAR BIOSCIENCE. - ISSN 1616-5187. - 10:8(2010), pp. 842-852.
Abstract:
Cell patterning is an important tool for organizing cells in surfaces and to reproduce in a simple way the tissue hierarchy and complexity of pluri-cellular life. The control of cell growth, proliferation and differentiation on solid surfaces is consequently important for prosthetics, biosensors, cell-based arrays, stem cell therapy and cell-based drug discovery concepts. We present a new electron beam lithography method for the direct and simultaneous fabrication of sub-micron topographical and chemical patterns, on a biocompatible and biodegradable PAA hydrogel. The localized e-beam modification of a hydrogel surface makes the pattern able to adsorb proteins in contrast with the anti-fouling surface. By also exploiting the selective attachment, growth and differentiation of PC12 cells, we fabricated a neural network of single cells connected by neuritis extending along microchannels. E-beam microlithography on PAA hydrogels opens up the opportunity of producing multifunctional microdevices incorporating complex topographies, allowing precise control of the growth and organization of individual cells.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Electron beam irradiation; Functionalization of polymers; Hydrogels; Networks; Proteins
Elenco autori:
LINO DOS REIS GABRIEL ARMANDO, FENILI FABIO, GIANFELICE ANTONELLA, G. Bongiorno, D. Marchesi, SCOPELLITI PASQUALE, A. Borgonovo, A. Podesta, INDRIERI MARCO, E. Ranucci, FERRUTI PAOLO, C. Lenardi, P. Milani
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