Leptin receptor expression and in vitro leptin actions on prostaglandin release and nitric oxide synthase activity in the rabbit oviduct
Articolo
Data di Pubblicazione:
2005
Citazione:
Leptin receptor expression and in vitro leptin actions on prostaglandin release and nitric oxide synthase activity in the rabbit oviduct / M. Zerani, C. Boiti, C. Dall'Aglio, L. Pascucci, M. Maranesi, G. Brecchia, C. Mariottini, G. Guelfi, D. Zampini, A. Gobbetti. - In: JOURNAL OF ENDOCRINOLOGY. - ISSN 0022-0795. - 185:2(2005), pp. 319-325.
Abstract:
In this study, we have examined the presence and the distribution of receptors for leptin (Ob-R) in the oviduct of rabbits, and the effects of leptin on the release of prostaglandin (PG) F2alpha and PGE2 and on the activity of nitric oxide (NO) synthase (NOS) by oviducts cultured in vitro. Rabbits were killed during the follicular phase and the oviducts were incubated in vitro with leptin, PGF2alpha, PGE2, NO donor and inhibitors of NOS and cyclo-oxigenase (COX). Using immunohistochemistry, Ob-R-like positive reaction was observed only in the cytoplasm of secretory cells, having stronger intensity in the infundibulum and ampulla tracts than in the isthmus. Both leptin and NO donor inhibited PGE2 release, whereas they enhanced PGF2alpha release; NOS inhibitor alone or with leptin increased PGE2 and decreased PGF2alpha production; NOS activity was enhanced by leptin, while PGs did not affect this enzyme. This study suggests that the oviduct could be a potential target for endocrine regulation by leptin, whose circulating levels may act as a metabolic signal modulating oviductal PG release through mediation of the NOS/NO system.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Rat oviduct; human endometrium; corpora-lutea; cyclooxygenase enzymes; dependent mechanism; reproductive axis; epithelial-cells; obese gene; hormone; growth
Elenco autori:
M. Zerani, C. Boiti, C. Dall'Aglio, L. Pascucci, M. Maranesi, G. Brecchia, C. Mariottini, G. Guelfi, D. Zampini, A. Gobbetti
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