Data di Pubblicazione:
2001
Citazione:
Structural determinants of CCR5 recognition and HIV-1 blockade in RANTES / V. Nardese, R. Longhi, S. Polo, F. Sironi, C. Arcelloni, R. Paroni, C. De Santis, P. Sarmientos, M. Rizzi, M. Bolognesi, V. Pavone, P. Lusso. - In: NATURE STRUCTURAL BIOLOGY. - ISSN 1072-8368. - 8:7(2001 Jul), pp. 611-615.
Abstract:
Certain chemokines act as natural antagonists of human immunodeficiency virus (HIV) by blocking key viral coreceptors, such as CCR5 and CXCR4, on the surface of susceptible cells. Elucidating the structural determinants of the receptor-binding and HIV-inhibitory functions of these chemokines is essential for the rational design of derivative molecules of therapeutic value. Here, we identify the structural determinants of CCR5 recognition and antiviral activity of the CC chemokine RANTES, showing that critical residues form a solvent-exposed hydrophobic patch on the surface of the molecule. Moreover, we demonstrate that the biological function is critically dependent on dimerization, resulting in the exposure of a large (180 Å2), continuous hydrophobic surface. Relevant to the development of novel therapeutic approaches, we designed a retroinverted RANTES peptide mimetic that maintained both HIV- and chemotaxis-antagonistic functions.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
V. Nardese, R. Longhi, S. Polo, F. Sironi, C. Arcelloni, R. Paroni, C. De Santis, P. Sarmientos, M. Rizzi, M. Bolognesi, V. Pavone, P. Lusso
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