Analysis of detergent-resistant membranes associated with apical and basolateral GPI-anchored proteins in polarized epithelial cells
Articolo
Data di Pubblicazione:
2006
Citazione:
Analysis of detergent-resistant membranes associated with apical and basolateral GPI-anchored proteins in polarized epithelial cells / S. Tivodar, S. Paladino, R. Pillich, A. Prinetti, V. Chigorno, G. van Meer, S. Sonnino, C. Zurzolo. - In: FEBS LETTERS. - ISSN 0014-5793. - 580:24(2006 Oct 16), pp. 5705-5712. [10.1016/j.febslet.2006.09.022]
Abstract:
Detergent-resistant membranes (DRMs) represent specialized membrane domains resistant to detergent extraction, which may serve to segregate proteins in a specific environment in order to improve their function. Segregation of glycosylphos-phatidylinositol-anchored proteins (GPI-APs) in DRMs has been shown to be involved in their sorting to the apical membrane in polarized epithelial cells. Nonetheless, we have shown that both apical and basolateral GPI-APs associate with DRMs. In this report we investigated the lipid composition of DRMs associated with an apical and a basolateral GPI-AP. We found that apical and basolateral DRMs contain the same lipid species although in different ratios. This specific lipid ratio is maintained after mixing the cells before lysis indicating that DRMs maintain their identity after Triton extraction.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Detergent-resistant membranes; Epithelial cells; Glycosylphosphatidylinositol-anchored proteins; Polarity; Rafts; Sorting
Elenco autori:
S. Tivodar, S. Paladino, R. Pillich, A. Prinetti, V. Chigorno, G. van Meer, S. Sonnino, C. Zurzolo
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