Identification and molecular characterization of Candidatus Phytoplasma mali isolates in north-western Italy
Articolo
Data di Pubblicazione:
2010
Citazione:
Identification and molecular characterization of Candidatus Phytoplasma mali isolates in north-western Italy / P. Casati, F. Quaglino, R. Tedeschi, F.M. Spiga, P. Spadone, A. Alma, P.A. Bianco. - In: JOURNAL OF PHYTOPATHOLOGY. - ISSN 0931-1785. - 158:2(2010 Feb), pp. 81-87.
Abstract:
Apple proliferation (AP) is an important disease and is
prevalent in several European countries. The causal
agent of AP is Candidatus Phytoplasma mali ( Ca.
Phytoplasma mali ). In this work, isolates of Ca.
Phytoplasma mali were detected and characterized
through polymerase chain reaction (PCR) and restriction
fragment length polymorphism (RFLP) analyses
of 16S rRNA gene and non-ribosomal DNA fragment.
The presence of three AP subtypes (AT-1, AT-2 and
AP-15) was identified in 31 symptomatic apple trees
and two samples each constituted by a pool of five
insects, collected in north-western Italy, where AT-1 is
a dominant subtype. Subsequent nucleotide sequence
analysis of the PCR-amplified 1.8 kb (P1 ⁄ P7) fragment,
containing the 16S rDNA, the 16S–23S
intergenic ribosomal region and the 5¢-end of the 23S
rDNA, revealed the presence of at least two phytoplasmal
genetic lineages within the AT-1 subtype, designed
AT-1a and AT-1b. Moreover, in silico single nucleotide
polymorphism (SNP) analysis based on 16S
rDNA sequence can differentiate AT-1 subtype from
AT-2 and AP-15 subtypes. Our data showed a high
degree of genetic diversity among Ca. Phytoplasma
mali population in north-western Italy and underlined
the possible use of the 16S rDNA analysis for the
identification and the geographical origin assignation
of isolates of AP phytoplasma. Molecular markers on
16S rDNA, here identified, could be useful for studying
the epidemiology of AP disease.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
'Candidatus Phytoplasma mali'; 16S rDNA; Apple proliferation; Genetic variability; Phytoplasmas; Single nucleotide polymorphisms
Elenco autori:
P. Casati, F. Quaglino, R. Tedeschi, F.M. Spiga, P. Spadone, A. Alma, P.A. Bianco
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