Data di Pubblicazione:
2007
Citazione:
Natural aging, expression of fibrosis-related genes and collagen deposition in rat lung / C. Calabresi, B. Arosio, L. Galimberti, E. Scanziani, R. Bergottini, G. Annoni, C. Vergani. - In: EXPERIMENTAL GERONTOLOGY. - ISSN 0531-5565. - 42:10(2007), pp. 1003-1011. [10.1016/j.exger.2007.06.016]
Abstract:
Aging lung is characterized by morpho-structural modifications, including progressive fibrosis, that lead to an altered function. Here we provide a comprehensive description of lung collagen expression and metabolism during natural aging of rats. Peribronchial collagen increased significantly in the oldest animals (p = 0.05 2- vs. 6- and 19-month-old rats), as a consequence of Collagen-I and Collagen-III (COL-I, COL-III) protein accumulation (p < 0.05 in 6-, 12- and 19-month-old rats versus the youngest). No changes in fibronectin (FN) protein expression and in COL-III and transforming grow factorβ-1 (TGFβ-1) mRNA expression were observed. Conversely the transcription activity of the COL-I gene was overexpressed in the oldest animals (p < 0.05). In the aged rats, the activity of lung matrix metalloproteinases (MMP), MMP-1 and MMP-2, dropped significantly (p < 0.05), whilst MMP-9 levels were slightly decreased. These changes were associated with a concomitant increase of tissue inhibitors of MMP (TIMP-1 and TIMP-2). All together, these results suggest that, during natural aging, collagen accumulation in the lung and its progressive fibrosis are mainly due to a reduced proteolytic activity of MMP, in which TIMP-1 and -2 seem to be the major regulating factors.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
C. Calabresi, B. Arosio, L. Galimberti, E. Scanziani, R. Bergottini, G. Annoni, C. Vergani
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