Data di Pubblicazione:
2008
Citazione:
Proteomic analysis of somatic embryogenesis in Vitis vinifera / M. Marsoni, M. Bracale,L. Espen, B. Prinsi, A. S. Negri, C. Vannini. - In: PLANT CELL REPORTS. - ISSN 0721-7714. - 27:2(2008), pp. 347-356.
Abstract:
Two dimensional gel electrophoresis coupled
to mass spectrometry has been used to study the somatic
embryogenesis in Vitis vinifera, by comparing embryogenic
and non embryogenic calluses of the Thompson
seedless cv. More than 1,000 spots were reproducibly
resolved in colloidal Coomassie brilliant blue stained gels
over a pI nonlinear range of 3–10 in the first dimension and
using homogeneous 12.5% polyacrylamide gels in the
second dimension. The expression pattern of 35 spots differed
significantly between the two samples. These spots
were processed by mass spectrometry analysis and the
protein identity was assigned by using both the nonredundant
protein and EST databases. Several responsive
proteins, some already known to be involved in the somatic
embryogenesis process while others, for the first time put
into relation with this process, have been described.
Moreover, they have been subdivided in functional categories,
and their putative role is discussed in terms of their
relevance in the somatic embryogenesis process.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Vitis vinifera ; Two-dimensional gel
electrophoresis ; Somatic embryogenesis ; Agricultural species ; Recalcitrant plant
Elenco autori:
M. Marsoni, M. Bracale,L. Espen, B. Prinsi, A. S. Negri, C. Vannini
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