Glutathione S-transferases in Festuca arundinacea : Identification, characterization and inducibility by safener benoxacor
Articolo
Data di Pubblicazione:
2007
Citazione:
Glutathione S-transferases in Festuca arundinacea : Identification,
characterization and inducibility by safener benoxacor / D. Del Buono, L. Scarponi, L. Espen. - In: PHYTOCHEMISTRY. - ISSN 0031-9422. - 68:21(2007 Jul 20), pp. 2614-2624.
Abstract:
Over recent years it has emerged how certain no crop-species can be employed in phytoremediating contaminated soils or preventing
herbicide pollution; in this contest Festuca arundinacea was investigated. Shoots of Festuca were submitted to fast protein liquid chromatography
in order to identify their glutathione S-transferases (GST; EC 2.5.1.18), by a combination of anionic, affinity and RP-HPLC
chromatography. The chromatographic procedure revealed satisfactory yield and four GSTs were identified: they were named FaGST I,
FaGST II, FaGST III and FaGST IV. Among these, significant differences were observed in the chromatographic behaviours, structure,
activity toward a ‘‘model’’ substrate, 1-chloro-2,4-dinitrobenzene, and responsiveness to the herbicide safener benoxacor. FaGST I
showed the highest activity toward the above substrate, and this activity was up-regulated by the herbicide safener. Therefore, FaGST
I was purified till homogeneity and was determined to be an heterodimer consisting of two subunits of 28.0 and 27.2 kDa. Each subunit
of FaGST I was further characterized by means of LC–ESI–MS/MS and immunoblotting analysis, which revealed that both the subunits
belong to the tau subclass.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
GSTs ; Festuca arundinacea ; herbicide ; herbicide safener
Elenco autori:
D. Del Buono, L. Scarponi, L. Espen
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