Prognostic role of non-neoplastic lymphocytes in lymph node aspirates from dogs with diffuse large B-cell lymphoma treated with chemo-immunotherapy
Articolo
Data di Pubblicazione:
2019
Citazione:
Prognostic role of non-neoplastic lymphocytes in lymph node aspirates from dogs with diffuse large B-cell lymphoma treated with chemo-immunotherapy / V. Martini, L. Aresu, F. Riondato, L. Marconato, M. Cozzi, D. Stefanello, S. Comazzi. - In: RESEARCH IN VETERINARY SCIENCE. - ISSN 0034-5288. - 125(2019), pp. 130-135. [10.1016/j.rvsc.2019.06.003]
Abstract:
Dogs with Diffuse Large B-Cell Lymphoma (DLBCL) benefit from the addition of active immunotherapy to traditional chemotherapy. We hypothesized that immune cells within neoplastic lymph nodes (LNs) may play a role in the tumor pathobiology and treatment response.
The present study describes the composition and prognostic role of non-neoplastic lymphocytes in LNs of 59 dogs with treatment-naive DLBCL receiving chemo-immunotherapy.
The percentage of small non-neoplastic cells and of CD5+, CD21+, CD4+ and CD8+ small cells was recorded via flow cytometry. CD4+/CD8+ and CD5+/large CD21+ cell ratios were calculated.
The likelihood of progression significantly diminished with increasing percentage of small cells, CD5+ and CD8+ small cells, and CD5+/large CD21+ cell ratio, with decreasing CD4+/CD8+ ratio and in non-anemic dogs.
Active immunotherapy is more effective in dogs with higher percentage of non-neoplastic lymphocytes at diagnosis. We lay the ground for future studies assessing the role of the immune system in the pathobiology of canine DLBCL.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Dog; DLBCLT-cells; Flow cytometry; Chemo-immunotherapy; Prognosis
Elenco autori:
V. Martini, L. Aresu, F. Riondato, L. Marconato, M. Cozzi, D. Stefanello, S. Comazzi
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