Nitric oxide production in living neurons is modulated by sphingosine: a fluorescence microscopy study
Articolo
Data di Pubblicazione:
2001
Citazione:
Nitric oxide production in living neurons is modulated by sphingosine: a fluorescence microscopy study / P. VIANI, P. GIUSSANI, A. FERRARETTO, A. SIGNORILE, L. RIBONI, G. TETTAMANTI. - In: FEBS LETTERS. - ISSN 0014-5793. - 506:3(2001), pp. 185-190. [10.1016/S0014-5793(01)02880-0]
Abstract:
An investigation was carried out into the possible effect of sphingosine (Sph) on nitric oxide (NO) production in living neurons. Differentiated granule cells were used in a dynamic videoimaging analysis of single cells labeled, simultaneously, with FURA-2 and the NO indicator 4,5-diaminofluorescein. The results demonstrate that Sph exerts a potent inhibitory effect on the Ca2+-dependent production of NO, without modifying the [Ca2+]i. The effect appears to be specific as neither ceramide nor Sph-1-phosphate had any effect on the NO and [Ca2+]i levels. The data demonstrate that Ca2+-dependent NO production is a specific Sph target in living granule cells, suggesting that this bioactive sphingoid plays a relevant role in neuronal NO signaling
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
nitric oxide ; sphingosine ; cerebellar granule cells ; dynamic videoimaging ; fluorescence indicator ; 4,5-diaminofluorescein
Elenco autori:
P. Viani, P. Giussani, A. Ferraretto, A. Signorile, L. Riboni, G. Tettamanti
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