Development of a peptide-containing chewing gum as a sustained release antiplaque antimicrobial delivery system
Articolo
Data di Pubblicazione:
2007
Citazione:
Development of a peptide-containing chewing gum as a sustained release antiplaque antimicrobial delivery system / J.A. Faraj, R. Dorati, A. Schoubeen, D. Worten, F. Selmin, Y. Capan, K. Leung, P.P. DeLuca. - In: AAPS PHARMSCITECH. - ISSN 1530-9932. - 8:1(2007), p. 26.26.
Abstract:
The objective of this study was to characterize the stability of KSL-W, an antimicrobial decapeptide shown to inhibit the growth of oral bacterial strains associated with caries development and plaque formation, and its potential as an antiplaque agent in a chewing gum formulation. KSL-W formulations with or without the commercial antibacterial agent cetylpyridinium chloride (CPC) were prepared. The release of KSL-W from the gums was assessed in vitro using a chewing gum apparatus and in vivo by a chew-out method. A reverse-phase high-performance liquid chromatography method was developed for assaying KSL-W. Raw material stability and temperature and pH effects on the stability of KSL-W solutions and interactions of KSL-W with tooth-like material, hydroxyapatite discs, were investigated.
KSL-W was most stable in acidic aqueous solutions and underwent rapid hydrolysis in base. It was stable to enzymatic degradation in human saliva for 1 hour but was degraded by pancreatic serine proteases. KSL-W readily adsorbed to hydroxyapatite, suggesting that it will also adsorb to the teeth when delivered to the oral cavity. The inclusion of CPC caused a large increase in the rate and extent of KSL-W released from the gums. The gum formulations displayed promising in vitro/in vivo release profiles, wherein as much as 90% of the KSL-W was released in a sustained manner within 30 minutes in vivo. These results suggest that KSL-W possesses the stability, adsorption, and release characteristics necessary for local delivery to the oral cavity in a chewing gum formulation, thereby serving as a novel antiplaque agent
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
KSL-W ; chewing gum ; sustained release ;
cetylpyridinium chloride ; antiplaque ; antimicrobial ; peptide ; stability
Elenco autori:
J.A. Faraj, R. Dorati, A. Schoubeen, D. Worten, F. Selmin, Y. Capan, K. Leung, P.P. DeLuca
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