Nitrogen Containing Bisphosphonates Impair the Release of Bone Homeostasis Mediators and Matrix Production by Human Primary Pre-Osteoblasts
Articolo
Data di Pubblicazione:
2019
Citazione:
Nitrogen Containing Bisphosphonates Impair the Release of Bone Homeostasis Mediators and Matrix Production by Human Primary Pre-Osteoblasts / C. Giannasi, S. Niada, D. Farronato, G. Lombardi, B. Manfredi, G. Farronato, A.T. Brini. - In: INTERNATIONAL JOURNAL OF MEDICAL SCIENCES. - ISSN 1449-1907. - 16:1(2019 Jan), pp. 23-32. [10.7150/ijms.27470]
Abstract:
Bisphosphonates (BPs) represent the first-line treatment for a wide array of bone disorders. Despite
their well-known action on osteoclasts, the effects they induce on osteoblasts are still unclear. In
order to shed light on this aspect we evaluated the impact of two nitrogen containing
bisphosphonates, Alendronate (ALN) and Zoledronate (ZOL), on human primary pre-osteoblasts.
At first, we showed an inhibitory effect on cell viability and alkaline phosphatase activity starting
from μM concentrations of both drugs. In addition, an inhibitory trend on mineralized nodules
deposition was observed. Then low doses of both ALN and ZOL rapidly increased the release of the
pro-inflammatory mediators TNFα and IL-1β, while increased DKK-1 and Sclerostin, both inhibitors
of osteoblastogenesis. Finally, ALN and 10-7M ZOL decreased the expression of type I Collagen and
Osteopontin, while both drugs slightly stimulated SPARC production. With these results, we would
like to suggest a direct inhibitory action on bone-forming cells by nitrogen containing
bisphosphonates.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
bisphosphonates, osteoblasts, alendronate, zoledronate
Elenco autori:
C. Giannasi, S. Niada, D. Farronato, G. Lombardi, B.P.E. Manfredi, G. Farronato, A.T.M. Brini
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