Data di Pubblicazione:
2017
Citazione:
Both genome and cytosol dynamics change in E. coli challenged with sublethal rifampicin / M. Wlodarski, B. Raciti, J. Kotar, M. Cosentino Lagomarsino, M. Gillian, P. Cicuta. - In: PHYSICAL BIOLOGY. - ISSN 1478-3967. - 14:1(2017 Feb 16), pp. 015005.1-015005.10.
Abstract:
While the action of many antimicrobial drugs is well understood at the molecular level, a systems-level physiological response to antibiotics remains largely unexplored. This work considers fluctuation dynamics of both the chromosome and cytosol in Escherichia coli, and their response to sublethal treatments of a clinically important antibiotic, rifampicin. We precisely quantify the changes in dynamics of chromosomal loci and cytosolic aggregates (a rheovirus nonstructural protein known as μNS-GFP), measuring short time-scale displacements across several hours of drug exposure. To achieve this we develop an empirical method correcting for photo-bleaching and loci size effects. This procedure allows us to characterize the dynamic response to rifampicin in different growth conditions, including a customised microfluidic device. We find that sub-lethal doses of rifampicin cause a small but consistent increase in motility of both the chromosomal loci and cytosolic aggregates. Chromosomal and cytosolic responses are consistent with each other and between different growth conditions.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Anti-Bacterial Agents, pharmacology; Chromosomes, Bacterial, drug effects, genetics; Escherichia coli, cytology, drug effects, genetics; Escherichia coli Infections, drug therapy, microbiology; Genome, Bacterial, drug effects; Humans; Rifampin, pharmacology
Elenco autori:
M. Wlodarski, B. Raciti, J. Kotar, M. Cosentino Lagomarsino, M. Gillian, P. Cicuta
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