Data di Pubblicazione:
2007
Citazione:
Wolbachia surface protein (WSP) inhibits apoptosis in human neutrophils / C. Bazzocchi, S. Comazzi, R. Santoni, C. Bandi, C. Genchi, M. Mortarino. - In: PARASITE IMMUNOLOGY. - ISSN 0141-9838. - 29:2(2007), pp. 73-79. [10.1111/j.1365-3024.2006.00915.x]
Abstract:
Polymorphonuclear cells (PMNs) are essential for the innate immune response
against invading bacteria. At the same time, modulation of PMNs' apoptosis or
cell death by bacteria has emerged as a mechanism of pathogenesis. Wolbachia
bacteria are Gram-negative endosymbionts of filarial nematodes and arthropods,
phylogenetically related to the genera Anaplasma, Ehrlichia and Neorickettsia
(family Anaplasmataceae). Although several pathogens are known to interfere with
apoptosis, there is only limited information on specific proteins that modulate
this phenomenon. This is the first evidence for the anti-apoptotic activity of a
surface protein of Wolbachia from filarial nematode parasites (the Wolbachia
surface protein, WSP). The inhibition of apoptosis was demonstrated on purified
human PMNs in vitro by different methods. TUNEL assay showed that the percentage
of dead cells was reduced after stimulation with WSP; Annexin V-FITC binding
assay confirmed that cell death was due mainly to apoptosis and not to necrosis.
Reduced caspase-3 activity in stimulated cells also confirmed an inhibition of
the apoptotic process.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Apoptosis; Filariasis; Immunopathology; PMN; Wolbachia surface protein
Elenco autori:
C. Bazzocchi, S. Comazzi, R. Santoni, C. Bandi, C. Genchi, M. Mortarino
Link alla scheda completa: