Validation of a paraoxon-based method for measurement of paraoxonase (PON-1) activity and establishment of RIs in horses
Articolo
Data di Pubblicazione:
2018
Citazione:
Validation of a paraoxon-based method for measurement of paraoxonase (PON-1) activity and establishment of RIs in horses / B. Ruggerone, F. Bonelli, I. Nocera, S. Paltrinieri, A. Giordano, M. Sgorbini. - In: VETERINARY CLINICAL PATHOLOGY. - ISSN 0275-6382. - 47:1(2018 Mar), pp. 69-77. [10.1111/vcp.12562]
Abstract:
Background: Paraoxonase-1 (PON-1) is an antioxidant compound that is considered
a negative acute phase protein. No information on the analytic performance of the
paraoxon method for measuring PON-1 in horse serum is available.
Objectives: The aim of this study was to validate a paraoxon-based method to
measure PON-1 in horses and to establish RIs in healthy horses and foals.
Methods: Horses and foals classified as healthy after physical examination and routine
biochemistry were used in the study. Serum PON-1 activity was measured with
an automated spectrophotometer and an enzymatic method validated in other species.
After the analytic validation (precision, accuracy, interference studies), RIs were
determined using the Reference Value Advisor software. The possible sex-, age-,
and breed-related differences were statistically investigated.
Results: The healthy study population included 120 horses and 55 foals. The paraoxon-
based method was precise (CVs < 4.0%) and accurate (P < .001 in linearity under
dilution and spike-recovery testing) but was affected by interference from mild bilirubinemia,
severe lipemia, and hemoglobinemia. The RIs recorded in the whole population
were 38.1–80.8 U/mL. According to the Harris and Boyd test, it would be advisable to
use separate RIs only for adult females and for Warmblood and Trotter adults.
Conclusions: This study demonstrated that the analytic performances of the
paraoxon-based method for measurement of PON-1 in horses are acceptable. The
PON-1 activity is lower in horses than in other domestic species. These results may
provide a basis for further studies designed to establish whether healthy and sick
horses can be correctly classified by using the PON-1 assay.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
acute phase proteins; equine; inflammation; oxidative stress
Elenco autori:
B. Ruggerone, F. Bonelli, I. Nocera, S. Paltrinieri, A. Giordano, M. Sgorbini
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