Phenotypic and functional characterization of mesenchymal stromal cells isolated from pediatric patients with severe idiopathic nephrotic syndrome
Articolo
Data di Pubblicazione:
2018
Citazione:
Phenotypic and functional characterization of mesenchymal stromal cells isolated from pediatric patients with severe idiopathic nephrotic syndrome / N. Starc, M. Li, M. Algeri, A. Conforti, L. Tomao, A. Pitisci, F. Emma, G. Montini, P. Messa, F. Locatelli, M.E. Bernardo, M. Vivarelli. - In: CYTOTHERAPY. - ISSN 1465-3249. - 20:3(2018 Mar), pp. 322-334. [10.1016/j.jcyt.2017.12.001]
Abstract:
Background: Idiopathic nephrotic syndrome (INS) is one of the most common renal diseases in the pediatric population; considering the role of the immune system in its pathogenesis, corticosteroids are used as first-line immunosuppressive treatment. Due to its chronic nature and tendency to relapse, a significant proportion of children experience co-morbidity due to prolonged exposure to corticosteroids and concomitant immunosuppression with second-line, steroid-sparing agents. Mesenchymal stromal cells (MSCs) are multipotent cells that represent a key component of the bone marrow (BM) microenvironment; given their unique immunoregulatory properties, their clinical use may be exploited as an alternative therapeutic approach in INS treatment. Methods: In view of the possibility of exploiting their immunoregulatory properties, we performed a phenotypical and functional characterization of MSCs isolated from BM of five INS patients (INS-MSCs; median age, 13 years; range, 11â16 years) in comparison with MSCs isolated from eight healthy donors (HD-MSCs). MSCs were expanded ex vivo and then analyzed for their properties. Results: Morphology, proliferative capacity, immunophenotype and differentiation potential did not differ between INS-MSCs and HD-MSCs. In an allogeneic setting, INS-MSCs were able to prevent both T- and B-cell proliferation and plasma-cell differentiation. In an in-vitro model of experimental damage to podocytes, co-culture with INS-MSCs appeared to be protective. Discussion: Our results demonstrate that INS-MSCs maintain the main biological and functional properties typical of HD-MSCs; these data suggest that MSCs may be used in autologous cellular therapy approaches for INS treatment.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
bone marrow niche; idiopathic nephrotic syndrome; immunomodulatory properties; mesenchymal stromal cells; immunology and allergy; immunology; oncology; genetics (clinical); cell biology; transplantation; cancer research
Elenco autori:
N. Starc, M. Li, M. Algeri, A. Conforti, L. Tomao, A. Pitisci, F. Emma, G. Montini, P. Messa, F. Locatelli, M.E. Bernardo, M. Vivarelli
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