Data di Pubblicazione:
2018
Citazione:
Measuring the levels of ribonucleotides embedded in genomic DNA / A. Meroni, M. Nava Giulia, S. Sertic, P. Plevani, M. Muzi-Falconi, F. Lazzaro (METHODS IN MOLECULAR BIOLOGY). - In: Genome Instability / [a cura di] M. Muzi-Falconi, G.W. Brown. - [s.l] : Humana Press Inc., 2018. - ISBN 9781493973057. - pp. 319-327 [10.1007/978-1-4939-7306-4_22]
Abstract:
Ribonucleotides (rNTPs) are incorporated into genomic DNA at a relatively high frequency during replication. They have beneficial effects but, if not removed from the chromosomes, increase genomic instability. Here, we describe a fast method to easily estimate the amounts of embedded ribonucleotides into the genome. The protocol described is performed in Saccharomyces cerevisiae and allows us to quantify altered levels of rNMPs due to different mutations in the replicative polymerase ε. However, this protocol can be easily applied to cells derived from any organism.
Tipologia IRIS:
03 - Contributo in volume
Keywords:
DNA polymerase; DNA repair; DNA replication; Genome stability; Genomic rNMPs; RNase H; Ribonucleotides incorporation
Elenco autori:
A. Meroni, M. Nava Giulia, S. Sertic, P. Plevani, M. Muzi-Falconi, F. Lazzaro
Link alla scheda completa:
Titolo del libro:
Genome Instability