Data di Pubblicazione:
2014
Citazione:
Peripheral nerve morphogenesis induced by scaffold micropatterning / F. Cerri, L. Salvatore, D. Memon, F. Martinelli Boneschi, M. Madaghiele, P. Brambilla, U. Del Carro, C. Taveggia, N. Riva, A. Trimarco, I.D. Lopez, G. Comi, S. Pluchino, G. Martino, A. Sannino, A. Quattrini. - In: BIOMATERIALS. - ISSN 0142-9612. - 35:13(2014), pp. 4035-4045. [10.1016/j.biomaterials.2014.01.069]
Abstract:
Several bioengineering approaches have been proposed for peripheral nervous system repair, with limited results and still open questions about the underlying molecular mechanisms. We assessed the biological processes that occur after the implantation of collagen scaffold with a peculiar porous micro-structure of the wall in a rat sciatic nerve transection model compared to commercial collagen conduits and nerve crush injury using functional, histological and genome wide analyses. We demonstrated that within 60 days, our conduit had been completely substituted by a normal nerve. Gene expression analysis documented a precise sequential regulation of known genes involved in angiogenesis, Schwann cells/axons interactions and myelination, together with a selective modulation of key biological pathways for nerve morphogenesis induced by porous matrices. These data suggest that the scaffold's micro-structure profoundly influences cell behaviors and creates an instructive micro-environment to enhance nerve morphogenesis that can be exploited to improve recovery and understand the molecular differences between repair and regeneration.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Biomaterials; Medical device; Nerve regeneration; Peripheral nervous system; Animals; Biocompatible Materials; Bioengineering; Female; Nerve Regeneration; Peripheral Nervous System; Rats; Rats, Sprague-Dawley; Tissue Scaffolds; Bioengineering; Ceramics and Composites; Biophysics; Biomaterials; Mechanics of Materials
Elenco autori:
C. Federica, S. Luca, M. Danish, F. Martinelli Boneschi, M. Marta, B. Paola, D.C. Ubaldo, T. Carla, R. Nilo, T. Amelia, L. Ignazio D., C. Giancarlo, P. Stefano, M. Gianvito, S. Alessandro, Q. Angelo
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