Purification to homogeneity of bovine prolactin by high-performance ion-exchange chromatography
Articolo
Data di Pubblicazione:
1991
Citazione:
Purification to homogeneity of bovine prolactin by high-performance ion-exchange chromatography / A. Berrini, V. Borromeo, C. Secchi. - In: JOURNAL OF CHROMATOGRAPHY A. - ISSN 1873-3778. - 547:1-2(1991), pp. 457-461.
Abstract:
Homogeneous bovine prolactin (bPRL) has been obtained using a procedure based on high-performance anion-exchange chromatography. The procedure enables up to 6 mg of 99.4% pure bPRL to be obtained per hour, with a recovery of 32.4%. The purity of the protein was checked by N-terminal sequencing and sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The highly purified bPRL obtained with this method is suitable for complete structural and immunochemical studies.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Growth-hormone; electrophoresis; variants; forms
Elenco autori:
A. Berrini, V. Borromeo, C. Secchi
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