Mycoplasma agalactiae MAG_5040 is a Mg2+-Dependent, Sugar-Nonspecific SNase Recognised by the Host Humoral Response during Natural Infection
Articolo
Data di Pubblicazione:
2013
Citazione:
Mycoplasma agalactiae MAG_5040 is a Mg2+-Dependent, Sugar-Nonspecific SNase Recognised by the Host Humoral Response during Natural Infection / C. Cacciotto, M.F. Addis, E. Coradduzza, L. Carcangiu, A.M. Nuvoli, G. Tore, G.M. Dore, D. Pagnozzi, S. Uzzau, B. Chessa, M. Pittau, A. Alberti. - In: PLOS ONE. - ISSN 1932-6203. - 8:2(2013), pp. e57775.1-e57775.11. [10.1371/journal.pone.0057775]
Abstract:
In this study the enzymatic activity of Mycoplasma agalactiae MAG_5040, a magnesium-dependent nuclease homologue to the staphylococcal SNase was characterized and its antigenicity during natural infections was established. A UGA corrected version of MAG_5040, lacking the region encoding the signal peptide, was expressed in Escherichia coli as a GST fusion protein. Recombinant GST-MAG_5040 exhibits nuclease activity similar to typical sugar-nonspecific endo- and exonucleases, with DNA as the preferred substrate and optimal activity in the presence of 20 mM MgCl2 at temperatures ranging from 37 to 45°C. According to in silico analyses, the position of the gene encoding MAG_5040 is consistently located upstream an ABC transporter, in most sequenced mycoplasmas belonging to the Mycoplasma hominis group. In M. agalactiae, MAG_5040 is transcribed in a polycistronic RNA together with the ABC transporter components and with MAG_5030, which is predicted to be a sugar solute binding protein by 3D modeling and homology search. In a natural model of sheep and goats infection, anti-MAG_5040 antibodies were detected up to 9 months post infection. Taking into account its enzymatic activity, MAG_5040 could play a key role in Mycoplasma agalactiae survival into the host, contributing to host pathogenicity. The identification of MAG_5040 opens new perspectives for the development of suitable tools for the control of contagious agalactia in small ruminants.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Amino Acid Sequence; Animals; Antigens, Bacterial; Cloning, Molecular; Computational Biology; Gene Expression Regulation, Bacterial; Goats; Magnesium; Micrococcal Nuclease; Molecular Sequence Data; Mycoplasma Infections; Mycoplasma agalactiae; Sequence Homology, Amino Acid; Sheep; Substrate Specificity; Immunity, Humoral; Agricultural and Biological Sciences (all); Biochemistry, Genetics and Molecular Biology (all); Medicine (all)
Elenco autori:
C. Cacciotto, M.F. Addis, E. Coradduzza, L. Carcangiu, A.M. Nuvoli, G. Tore, G.M. Dore, D. Pagnozzi, S. Uzzau, B. Chessa, M. Pittau, A. Alberti
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