A human gut metaproteomic dataset from stool samples pretreated or not by differential centrifugation
Articolo
Data di Pubblicazione:
2015
Citazione:
A human gut metaproteomic dataset from stool samples pretreated or not by differential centrifugation / A. Tanca, A. Palomba, S. Pisanu, M.F. Addis, S. Uzzau. - In: DATA IN BRIEF. - ISSN 2352-3409. - 4(2015 Sep), pp. 559-562.
Abstract:
We present a human gut metaproteomic dataset deposited in the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD001573. Ten aliquots of a single stool sample collected from a healthy human volunteer were either pretreated by differential centrifugation (DC; N=5) or not centrifuged (NC; N=5). Protein extracts were then processed by filter-aided sample preparation, single-run liquid chromatography and high-resolution mass spectrometry, and peptide identification was carried out using Sequest-HT as search engine within the Proteome Discoverer informatic platform. The dataset described here is also related to the research article entitled "Enrichment or depletion? The impact of stool pretreatment on metaproteomic characterization of the human gut microbiota" published in Proteomics (Tanca et al., 2015), [1].
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
differential centrifugation; gut microbiome; human gut; metaproteomics; sample preparation; 3304; multidisciplinary
Elenco autori:
A. Tanca, A. Palomba, S. Pisanu, M.F. Addis, S. Uzzau
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