Data di Pubblicazione:
2017
Citazione:
TOWARDS THE USE OF NEW TECHNOLOGIES FOR NUTRITIONAL SAFETY / A. Pilotto ; SAVOINI, GIOVANNI. DIPARTIMENTO DI SCIENZE VETERINARIE PER LA SALUTE, LA PRODUZIONE ANIMALE E LA SICUREZZA ALIMENTARE, 2017 Apr 11. 29. ciclo, Anno Accademico 2016. [10.13130/pilotto-adriano_phd2017-04-11].
Abstract:
In this thesis five experimental studies were reported with the objective to improve the knowledge and provide new solutions for different and important aspects of dairy cow nutrition. The first trial was performed to examine the relationships between blood concentrations of fatty acids (NEFA), β-hydroxybutyrate (BHB) and α-tocopherol during the periparturient period in dairy cows. For the study 131 cows from 4 different farms, 2 in Italy and 2 in Greece were used. We determined blood concentrations of NEFA, BHB, and α- tocopherol at dry-off, at calving, and 30d postpartum. We found a weak correlation between NEFA and BHB throughout the periparturient period. Negative correlations between NEFA and α-tocopherol were highly significant at 30d postpartum and approached the level of
significance at dry-off. However, both correlations became nonsignificant following the adjustment of α-tocopherol with cholesterol, indicating that the correlations were a reflection
of changes in lipid transport. We found significant negative correlations between BHB and α- tocopherol after adjustment with cholesterol. The aims of the second research presented was to understand the effects of lactic acid bacteria (LAB) inoculation on fermentation products and quality of alfalfa and ryegrass silage and the use of thermal camera and electronic nose to evaluate the silage quality. Wilted ryegrass and alfalfa silage were inoculated with a commercial product containing Lactobacillus Rhamnosus and Lactobacillus Farciminis and stored in triplicate micro fermenters for each treatment. The silos were opened after 2, 5, 30, 60, 90 and 120 days and silages were collected and analyzed. The results obtained showed better acidification of treated silages with pH value lower in both alfalfa and ryegrass silages (P≤0.01). Alfalfa silage has had higher content of lactic acid (P≤0.01) with unexpected higher values of NH4 (P≤0.01). In ryegrass silage the LAB concentration was lower in treated compared to control silage (P≤0.01). Instead no significant difference of LAB concentration was observed between control and treated alfalfa silages. Strong negative correlation between pH and lactic acid 97% (P≤0.01) and positive correlation between pH and LAB concentration 93% (P≤0.01) were observed in alfalfa silage. The LAB concentration was positive correlated with pH value 94% (P≤0.01). Significant but not strong negative correlation was observed between pH and NH4 33% (P≤0.05). Probably due to the
good quality of both treated and control silages no differences of temperature were observed. Although, different results were obtained between ryegrass and silage analysis the electronic nose showed promising ability to evaluate the silage quality but further studies will be needed to understand his ability to evaluate the silage quality.
In the third experimental trial the combined use of thermal camera and electronic nose was evaluated for rapid assessment of unfavorable fermentations that can occur in a corn silage mass. From April to September, five sampling times were performed to collect silage samples. The thermal camera was used to detect the temperature. For all samples chemical analysis were performed to evaluate the silage quality and was used electronic nose to perform the off-flavors analysis. The silage quality remained constant and no differences were observed throughout the trial. Significant statistical differences were observed between samples with normal temperature and that with higher temperature for pH value (P<0.01), lactic acid (P
Tipologia IRIS:
Tesi di dottorato
Elenco autori:
A. Pilotto
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