Gastrointestinal digestates of Grana Padano and Trentingrana cheeses promote intestinal calcium uptake and extracellular bone matrix formation in vitro
Articolo
Data di Pubblicazione:
2016
Citazione:
Gastrointestinal digestates of Grana Padano and Trentingrana cheeses promote intestinal calcium uptake and extracellular bone matrix formation in vitro / P. De Luca, S. Bruschi, M. Maggioni, M. Stuknytė, S. Cattaneo, M. Bottani, A. Fiorilli, F. Rossi, I. De Noni, A. Ferraretto. - In: FOOD RESEARCH INTERNATIONAL. - ISSN 0963-9969. - 89(2016 Nov), pp. 820-827. [10.1016/j.foodres.2016.10.008]
Abstract:
In the present work, Grana Padano (GP) and Trentingrana (TN) cheeses at different ripening time were in vitro
digested. To study calcium uptake and utilization, the intact digestates (selected doses that do not alter cell via-
bility and Transepithelial Electrical Resistance) were administered to Caco2/HT-29 70/30 cells, cultured on a
semipermeable membrane in transwells, as a model of human intestinal epithelium. Intact digestates as well
as the whole basolateral solutions (mimicking the passage of digestates through intestinal cells before reaching
the blood flow and bone) in parallel were further administered to human osteoblast-like cells SaOS-2 to study the
extracellular bone matrix formation. In vitro digestates deriving from GP and TN promoted calcium uptake and
extracellular bone matrix formation independently of both the cheese type and its ripening period (13, 19 or
26 months). The present study reports the ability of whole digestates of GP and TN cheeses to improve intestinal
calcium absorption and bone matrix formation in vitro. Once fully explored at bone level, this finding could better
support the role of cheese in ameliorating calcium deficiencies and associated diseases in vivo.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
cone cells; calcium; casein phosphopeptides; cheese digestate; Co-culture cells
Elenco autori:
P. De Luca, S. Bruschi, M. Maggioni, M. Stuknytė, S. Cattaneo, M. Bottani, A. Fiorilli, F. Rossi, I. De Noni, A. Ferraretto
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