Rapid determination of p-aminohippuric acid in serum and urine by high-performance liquid chromatography
Articolo
Data di Pubblicazione:
1997
Citazione:
Rapid determination of p-aminohippuric acid in serum and urine by high-performance liquid chromatography / R. Marsilio, R. Dall'Amico, G. Montini, L. Murer, M. Ros, G. Zacchello, F. Zacchello. - In: JOURNAL OF CHROMATOGRAPHY. B, BIOMEDICAL APPLICATIONS. - ISSN 1572-6495. - 704:1-2(1997 Dec), pp. 359-364.
Abstract:
We report a simple and rapid HPLC procedure for measuring p-aminohippuric acid in serum and urine. After deproteinization with acetonitrile and addition of p-aminobenzoic acid as an internal standard, the chromatographic run is performed on a C18 column with the absorbance detector set at 275 nm. The separation is carried out in 10 min at a flow-rate of 1.0 ml/min with a mobile phase composed of 7 mmol/l l-decanesulfonic acid, pH 3.70, and acetonitrile (82:18, v/v). The relationship between p-aminohippuric acid concentration and the p-aminohippuric acid/internal standard peak area is linear up to 100 μg/ml. Within-run precision measured at three different p-aminohippuric acid concentrations ranges from 1.73 to 1.98% in serum and from 0.72 to 1.32% in urine. Between-run precision varies from 1.80 to 3.06% in serum and from 1.05 to 2.66% in urine. Analytical recovery is between 98.26 and 99.44% in serum and from 99.57 to 100.45% in urine. The method is very simple, sensitive, and requires small volumes of sample for the assay (100 μl). Therefore, it could be a useful tool for the analysis of p-aminohippuric acid in the evaluation of renal plasma flow.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
p-aminohippuric acid
Elenco autori:
R. Marsilio, R. Dall'Amico, G. Montini, L. Murer, M. Ros, G. Zacchello, F. Zacchello
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