Data di Pubblicazione:
2016
Citazione:
With or without you - Proteomics with or without major plasma/serum proteins / E. Gianazza, I. Miller, L. Palazzolo, C. Parravicini, I. Eberini. - In: JOURNAL OF PROTEOMICS. - ISSN 1874-3919. - 140(2016 May), pp. 62-80. [10.1016/j.jprot.2016.04.002]
Abstract:
The first sections of this review compile and discuss strategies and protocols for managing plasma/serum as a source of biomarkers relevant to human disease. In many such cases, depletion of abundant protein(s) is a crucial preliminary step to the procedure; specific conceptual and technical approaches, however, make it possible to effectively use to this purpose whole plasma/serum. The final sections focus instead on the complexity associated with each of the major serum/plasma proteins in terms of both, multiple molecular structures (existence of a number of protein species) and of multiple molecular functions (behavior as multifunctional/multitasking/moonlighting proteins). Reviewing evidence in these and some related fields (regulation of the synthetic pattern by proteins and non-protein compounds and its connection with health and disease) prompts the suggestion/recommendation that information on the abundant components of plasma/serum proteome is routinely obtained and processed/mined as a valuable contribution to the characterization of any non-physiological condition and to the understanding of its mechanisms and of its implications/sequels.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Depletion; Enrichment; Immunodepletion; Protein species; Serum proteins; Biochemistry; Biophysics
Elenco autori:
E. Gianazza, I. Miller, L. Palazzolo, C. Parravicini, I. Eberini
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