Colorazione multipla fluorescente e sistema "CASA" degli spermatozoi di suino : valutazione della loro vitalità in diluitori a breve e lunga conservazione
Articolo
Data di Pubblicazione:
2008
Citazione:
Colorazione multipla fluorescente e sistema "CASA" degli spermatozoi di suino : valutazione della loro vitalità in diluitori a breve e lunga conservazione / V. Maggio, A. Lange Consiglio, P. Bassini, L. Gottardi, F. Cremonesi. - In: RIVISTA DI SUINICOLTURA. - ISSN 0035-662X. - 49:4(2008 Apr), pp. 143-152.
Abstract:
The aim of this study was to test a simple
and consistent method for the simultaneous
evaluation of the integrity of the
plasma, the acrosome and the mitochondrial
sheath membranes of boar spermatozoa
extended in six short-term (A-F)
and three long-term (G-I) commercial extenders.
Sperm samples collected from three
boars of proven fertility were evaluated
examining motility by Computer Assisted
Sperm Analyzer (CASA), viability using
Propidium Iodide (PI) staining, acrosome
intactness by fluorescein isothiocyanate-
conjugated peanut agglutin (FITCPNA)
staining and mithocondrial activity
by 5,5’, 6,6’-tetrachloro-1,1’, 3,3’ tetraethylbenzimidazolyl-
carbocyanine iodide
(JC-1) staining. The results obtained
from the fluorescent multiple staining,
were compared with sperm motility assessed
by CASA and with sperm morphology.
Sperm evaluations along with bacteriological
contamination (CFU) were
performed during a 6-12 days period, depending
on the labelled preservation period
of each extender.
In our study it was found that the frequency
of viable spermatozoa with nonreacted
acrosome and intact mitochondria
was positively correlated with the rate
of motile spermatozoa (r2>0.9) regardless
of the extender used.
In all extender the frequency of motile
spermatozoa significantly decreased
with the preservation period (p<0.05).
The rate of progressively motile spermatozoa
was very low in all the assayed extenders
(14,95% ±0,89 since day 0) but
this motility could have been influenced
by the diluition effect.
The fluorescent multiple staining test is a
potent indicator of sperm motility because
it analyses the mitochondrial integrity
independently from observable alteration
of motility and our results indicated
that the low progressive motility was only
apparent since the mitochondrial sheath
of most spermatozoa was intact.
CFU number remained stable during the
study period for all extenders and there
was no significant correlation between
CFU, percentage of motility and dead
spermatozoa.
In conclusion, the integration of the data
obtainable from the fluorescent multiple
staining test and the CASA analysis are
required for a correct evaluation of boar
sperm viability during preservation.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
spermatozoi; vitalità; diluitori a breve e lunga conservazione; CASA; colorazione multipla fluorescente
Elenco autori:
V. Maggio, A. Lange Consiglio, P. Bassini, L. Gottardi, F. Cremonesi
Link alla scheda completa: