In vitro inhibition of human cGMP-specific phosphodiesterase-5 by polyphenols from red grapes
Articolo
Data di Pubblicazione:
2005
Citazione:
In vitro inhibition of human cGMP-specific phosphodiesterase-5 by polyphenols from red grapes / M. Dell'Agli, G.V. Galli, U. Vrhovsek, F. Mattivi, E. Bosisio. - In: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. - ISSN 0021-8561. - 53:6(2005), pp. 1960-1965.
Abstract:
A moderate consumption of red wine may reduce the risk of cardiovascular diseases via wine-derived phenolic compounds. A variety of biological mechanisms have been proposed for wine-derived phenolic compounds including nitric oxide-mediated vasorelaxation. This study examined whether the vasodilating effect of wine-derived phenolic compounds was associated with the inhibition of phosphodiesterases (PDEs) and, in particular, PDE5. For this purpose, human recombinant PDE5A1 isoform was prepared by expression of the full-length cDNA of PDE5A1 into COS-7 cells. Red wine and the extracts from grape skin inhibited PDE5A1 activity, whereas the seed extracts had a negligible effect. The mixture of anthocyanins inhibited the enzyme activity (IC50 = 11.6 microM), with malvidin-3-O-beta-glucoside (IC50 = 35.4 microM) and malvidin (IC50 = 24.9 microM) the most potent among the monoglucosides and aglycons, respectively. trans-Resveratrol and trans-piceid exhibited negligible effects, whereas hydroxycinnamates were completely inactive. These results indicate that polyphenols-induced vasorelaxation may also be sustained by smooth muscle PDE inhibition by anthocyanins present in red wines and grapes
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Anthocyanins; Phosphodiesterase-5; Red grape polyphenols; Vitis vinifera; Wine
Elenco autori:
M. Dell'Agli, G.V. Galli, U. Vrhovsek, F. Mattivi, E. Bosisio
Link alla scheda completa: