Data di Pubblicazione:
2007
Citazione:
Excitotoxicity-related endocytosis in cortical neurons / A. Vaslin, J. Puyal, T. Borsello, P.G.H. Clarke. - In: JOURNAL OF NEUROCHEMISTRY. - ISSN 0022-3042. - 102:3(2007), pp. 789-800.
Abstract:
Recent studies showed that endocytosis is enhanced in neurons exposed to an excitototoxic stimulus. We here confirm and analyze this new phenomenon using dissociated cortical neuronal cultures. NMDA-induced uptake (FITC-dextran or FITC or horseradish peroxidase) occurs in these cultures and is due to endocytosis, not to cell entry through damaged membranes; it requires an excitotoxic dose of NMDA and is dependent on extracellular calcium, but occurs early, while the neuron is still intact and viable. It involves two components, NMDA-induced and constitutive, with different characteristics. Neither component involves specific binding of the endocytosed molecules to a saturable receptor. Strikingly, molecules internalized by the NMDA-induced component are targeted to neuronal nuclei. This component, but not the constitutive one, is blocked by a c-Jun N-terminal protein kinase inhibitor. In conclusion, an excitotoxic dose of NMDA triggers c-Jun N-terminal protein kinase-dependent endocytosis in cortical neuronal cultures, providing an in vitro model of the excitotoxicity-induced endocytosis reported in intact tissues.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
c-Jun N-terminal kinase; dextran; D-JNKI1; neurons; NMDA; primary culture
Elenco autori:
A. Vaslin, J. Puyal, T. Borsello, P.G.H. Clarke
Link alla scheda completa: