Magnetic resonance imaging visualization of vulnerable atherosclerotic plaques at the brachiocephalic artery of apolipoprotein E knockout mice by the blood-pool contrast agent B22956/1
Articolo
Data di Pubblicazione:
2014
Citazione:
Magnetic resonance imaging visualization of vulnerable atherosclerotic plaques at the brachiocephalic artery of apolipoprotein E knockout mice by the blood-pool contrast agent B22956/1 / C. Parolini, M. Busnelli, G.S. Ganzetti, F. Dellera, S. Manzini, E. Scanziani, J.L. Johnson, C.R. Sirtori, G. Chiesa. - In: MOLECULAR IMAGING. - ISSN 1535-3508. - 13:5(2014), pp. 00012.1-00012.9. [10.2310/7290.2014.00012]
Abstract:
The aim of this study was to identify, by magnetic resonance imaging (MRI), the ability of the blood-pool contrast agent B22956/1 to detect atherosclerotic plaques developing at the brachiocephalic artery of apolipoprotein E knockout (apoE-KO) mice and to possibly identify vulnerable atherosclerotic lesions. After high-fat feeding for 8 or 12 weeks, MRIs of brachiocephalic arteries were acquired before and after B22956/1 administration; then vessels were removed and analyzed by histology. B22956/1 injection caused a rapid increase in plaque signal enhancement and plaque to muscle contrast values, which remained stable up to 70 minutes. A linear correlation between signal enhancement and macrophage content was found 10 minutes after B22956/1 injection (p < .01). Signal enhancement and plaque to muscle contrast values correlated with macrophage content 40 minutes after contrast agent administration (p < .01). Finally, 70 minutes after B22956/1 infusion, plaque to muscle contrast significantly correlated with the percentage of stenosis (p < .005). B22956/1 administration to high fat-fed apoE-KO mice resulted in a rapid enhancement of atherosclerotic plaques and in a great ability to rapidly visualize vulnerable plaques, characterized by a high macrophage content. These results suggest that B22956/1 could represent an interesting tool for the identification of atherosclerotic plaques potentially leading to acute cardiovascular events.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Animals; Apolipoproteins E; Brachiocephalic Trunk; Contrast Media; Diet, High-Fat; Magnetic Resonance Imaging; Male; Mice; Mice, Knockout; Organometallic Compounds; Plaque, Atherosclerotic
Elenco autori:
C. Parolini, M. Busnelli, G.S. Ganzetti, F. Dellera, S. Manzini, E. Scanziani, J.L. Johnson, C.R. Sirtori, G. Chiesa
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