Data di Pubblicazione:
2015
Citazione:
New function of Superoxide dismutase 1 in the nuclear compartment / C. Cereda, O. Pansarasa, S. Gagliardi, M. Dell’Orco, M. Bordoni, S.L. Salvia, L. Diamanti, E. Maffioli, G. Tedeschi, M. Ceroni. ((Intervento presentato al 46. convegno Congress of the Italian Neurological Society tenutosi a Genova nel 2015.
Abstract:
Background: Recent evidences showed that, in PBMCs from sALS patients, there is an over-expression of SODJ mRNA (1). which in contrast with the uoehanged cytopla.•mic level of the protein [2). nus discrepancy could be explained considering' a re-Iocalization of Ute "nlissing" protein in the nucleus. Mnreovec, in PBMCs of sALS patienls. SOD 1 minslocates from the cytopl&sm to the nuclear com• partrncntin stressful conditions [3J. Objective.~: The exact role of SODJ in the nuclear oompanmenr remains a critical issue to clarify. Thus, we aim to investigate whether and how nuclear $001 COUid XCl ag.ainst oxidative SttCSS holh itl 1\eO• IOb!IUtolJla cell line SHSY5Y, a celluler model of nearodegeneratio», and in PBMCs of sALS patients.
Materials and melh(>ds: SOD! tocatizauon in SHSYSY and in ,ALS patients was investic.itcd by both WB and immnDC>fluOtCSCcnce.
By means Mass Spectrometry (MS) we searched for the modifications ttl!O\ving for SOD I transtocation snd by lmmunop:ecipitation (IP) we identify the binding protein involved in
Tipologia IRIS:
14 - Intervento a convegno non pubblicato
Elenco autori:
C. Cereda, O. Pansarasa, S. Gagliardi, M. Dell’Orco, M. Bordoni, S.L. Salvia, L. Diamanti, E. Maffioli, G. Tedeschi, M. Ceroni
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