Data di Pubblicazione:
2014
Citazione:
Skin proteomic comparison among four grape cultivars with different anthocyanin contents / A.S. Negri, B. Prinsi, O. Failla, A. Scienza, M. Cocucci, L. Espen. - In: ACTA HORTICULTURAE. - ISSN 0567-7572. - 1046(2014 Jul 20), pp. 685-693. ((Intervento presentato al 10. convegno International Conference on Grapevine Breeding and Genetics tenutosi a Geneva nel 2010 [10.17660/ActaHortic.2014.1046.95].
Abstract:
Grape skin is characterized by a very intense metabolism during ripening
involving many biosynthetic activities such as those relating to secondary
compounds like the anthocyanins. In order to improve our knowledge about the
physiology of this tissue, we performed a comparison among the proteomes of the
ripe skin of ‘Riesling Italico’, ‘Pinot Gris’, ‘Pinot Noir’ and ‘Croatina’, genotypes
that accumulate anthocyanins to different extents. In order to examine quantitative
changes in proteomic maps, data underwent a two-way ANOVA test using cultivars
and years as factors. The 297 significant variations underwent two different
clustering procedures. By means of the two-way hierarchical clustering, it was
possible to observe that the gels of the four cultivars were arranged in an order that
clearly reflected anthocyanin levels and emphasized the similarities between the two
Pinots, from one side, and the divergence of the proteome of ‘Croatina’, from the
other. It was then possible to group the 297 spots, according to their trends, in six
clusters through the k-means procedure. One of the clusters included 85 spots
showing a variation profile that could be associated with anthocyanin levels. Some
were identified through LC-ESI-MS/MS and their role was discussed.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Anthocyanin; Skin; Two-dimensional electrophoresis
Elenco autori:
A.S. Negri, B. Prinsi, O. Failla, A. Scienza, M. Cocucci, L. Espen
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