Data di Pubblicazione:
2015
Citazione:
Characterization of thymus atrophy in calves with subclinical BVD challenged with BHV-1 / F. Romero-Palomo, M.A. Risalde, V. Molina, S. Lauzi, M.J. Bautista, J.C. Gómez-Villamandos. - In: VETERINARY MICROBIOLOGY. - ISSN 0378-1135. - 177:1-2(2015 May), pp. 32-42. [10.1016/j.vetmic.2015.02.018]
Abstract:
Since the thymus is a target organ for the bovine viral diarrhea virus (BVDV), our experiment aimed to understand its relationship with the immunosuppressive effect by studying the consequences of a previous infection with BVDV on the thymus of calves challenged with bovine herpesvirus 1.1 (BHV-1). For this purpose, 12 animals were inoculated intranasally with non-cytopathic BVDV-1; 12 days later, 10 of them were coinfected intranasally with BHV-1. These animals were euthanized in batches of two at 0, 1, 2, 4, 7 or 14 dpi with BHV-1. Another 10 calves were inoculated solely with BHV-1 and euthanized in batches of two at 1, 2, 4, 7 or 14 dpi with BHV-1; two uninoculated calves were used as negative controls. Thymus samples from these animals were processed for viral detection and histopathological, immunohistochemical, and ultrastructural studies focused on BVDV/BHV-1 antigens, cortex:medulla ratio, apoptosis (TUNEL and caspase-3), collagen deposition, and factor VIII endothelial detection. Our study revealed the immunohistochemical presence of BVDV antigen in all animals in the BVDV-infected group, unlike BHV-1 detection, which was observed in animals in both infection groups only by molecular techniques. BVDV-preinfected animals showed severe atrophic changes associated with reduced cortex:medulla ratio, higher presence of cortical apoptosis, and increased collagen deposition and vascularization. However, calves solely infected with BHV-1 did not show atrophic changes. These findings could affect not only the numbers of circulating and local mature T cells but also the T cell-mediated immunity, which seems to be impaired during infections with this virus, thus favoring pathogenic effects during secondary infections.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Bovine viral diarrhea virus (BVDV); Bovine herpesvirus type 1 (BHV-1); Thymus atrophy; Apoptosis; Immunohistochemistry
Elenco autori:
F. Romero Palomo, M.A. Risalde, V. Molina, S. Lauzi, M.J. Bautista, J.C. Gómez Villamandos
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