Data di Pubblicazione:
1994
Citazione:
Metabolic suffering in Actinidia deliciosa callus growing medium / A. Abruzzese, C. Alisi, L. Espen, S. Morgutti, N. Negrini, G.A. Sacchi, M. Cocucci, S. Cocucci, A.R. Leva - In: Abstract International Congress of Plant Tissue and Cell Culture[s.l] : Congress of Plant Tissue and Cell Culture, 1994. - pp. 232-232 (( Intervento presentato al VIII. convegno International Congress of Plant Tissue and Cell Culture tenutosi a Firenze nel 1994.
Abstract:
Calluses of Actinidia deliciosa wcre grown in successive 45-days
subcu[tures in Gambors as medium (20g/l sucrosese). In order to evaluate
the changes that take place during tbe growth of the callus, we measured ,
at different times (10, 35 and 45 days), the incease in fresh weight, the
respiratory activity, the levels of inorganic caltions (K+' Na+, Ca2+ and
Mg2+) and of some metabolites (sucrose, giucose-6-P, malicc acid. amino
acids and adenylic nudeotides). After an cxponential growing phase (0-
10 days), the callus growth rate became practically constant up (0 the
45th day. In the first period the anaIyzed metabolic parameters reached
levels that remained pratically constant up the 35~ day. In the last period
the level level and the respiration rate greatly decreased while the
ATP/ADP ratio increased; K+, Ca2 + and Mg2+. slightly decreased while
Na + greatly increased. Upon transfer onto fresh medium, the above cited
parameters returned 10 the former levels, suggesling prompt recovery of
the calluses.
It is interesting to point oul that in the last period of incubation important
metabolic changes take place. even if the growth rate is not affected. This
might play an important role in micropropagation and selection of the
cultures, and should be taken into account in the: setting up of the culture
prococols.
Tipologia IRIS:
03 - Contributo in volume
Elenco autori:
A. Abruzzese, C. Alisi, L. Espen, S. Morgutti, N. Negrini, G.A. Sacchi, M. Cocucci, S. Cocucci, A.R. Leva
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Titolo del libro:
Abstract International Congress of Plant Tissue and Cell Culture