Evaluation of C-reactive protein (CRP) as clinical biomarker in naturally heartworm infected dogs : field study
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Data di Pubblicazione:
2013
Citazione:
Evaluation of C-reactive protein (CRP) as clinical biomarker in naturally heartworm infected dogs : field study / L. Venco, W. Bertazzolo, G. Giordano, S. Paltrinieri. ((Intervento presentato al convegno Triennial Heartworm Symposium tenutosi a New Orleans nel 2013.
Abstract:
C-reactive protein (CRP) is a protein found in the blood, the levels of which rise in response to inflammation and is considered an acute-phase protein. Its physiological role is to bind to phosphocholine expressed on the surface of dead or dying cells (and some types of bacteria) in order to activate the complement system via the C1Q complex. CRP is synthesized by the liver and his increment is due to a rise in the plasma concentration of cytokines such as interleukin-6 ( IL-6), which is produced predominantly by macrophages as well as adipocytes and Tumor necrosis factor-alpha (TNF α). The acute phase response develops in a wide range of acute and chronic inflammatory conditions like infections; and other inflammatory diseases; malignancy; and tissue injury or necrosis. These conditions cause release of interleukin-6 and other cytokines that trigger the synthesis of CRP and fibrinogen by the liver. During the acute phase response, levels of CRP rapidly increase and with resolution of the acute phase response, CRP declines with a relatively short half-life. Measuring CRP level is a screen for a wide range of diseases. Rapid, marked increases in CRP occur with inflammation, infection, trauma and tissue necrosis, malignancies, and autoimmune disorders. Because there are a large number of disparate conditions that can increase CRP production, an elevated CRP level does not diagnose a specific disease. The physiological role of CRP is to bind to phosphocholine expressed on the surface of dead or dying cells (and some types of bacteria) in order to activate the complement system. CRP binds to phosphocholine on microbes and damaged cells and enhances phagocytosis by macrophages. Thus, CRP participates in the clearance of necrotic and apoptotic cells. Arterial damage results from white blood cell invasion and inflammation within the wall may create an increasing of CRP such as in deep vein thrombosis in human beings. The aim of this study was to investigate if CRP measuring (once or sequential) may have a diagnostic or prognostic value in Heartworm disease or if it may use for staging and monitoring the disease in dogs, and to discover if increasing of CRP is related to worm burden or lung or arterial damage.
Sera for evaluating CRP values were collected from different groups of dogs:
- Normal dogs heartworm free.
- Dogs , heartworm free , with congenital or acquired cardiac disease affecting lungs blood circulation.
- Dogs Heartworm infected in different stages of the disease.
- Dogs Heartworm naturally infected randomly chosen from a population with different severity of symptoms related to the infection.
Results shows that CRP levels increase in Heartworm infected dogs and that the level of increasing seems to be mainly correlated to the severity of pulmonary arterial damage or thromboembolism .
Measuring CRP levels could therefore be considered a useful, cheap and simple Biomarker for staging the disease , monitoring therapy or decide in clinical trials which kind of therapy has advantages
Tipologia IRIS:
14 - Intervento a convegno non pubblicato
Elenco autori:
L. Venco, W. Bertazzolo, G. Giordano, S. Paltrinieri
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