Marine microorganisms as source of stereoselective esterases and ketoreductases : kinetic resolution of a prostaglandin intermediate
Articolo
Data di Pubblicazione:
2015
Citazione:
Marine microorganisms as source of stereoselective esterases and ketoreductases : kinetic resolution of a prostaglandin intermediate / V. De Vitis, B. Guidi, M.L. Contente, T. Granato, P. Conti, F. Molinari, E. Crotti, F. Mapelli, S. Borin, D. Daffonchio, D. Romano. - In: MARINE BIOTECHNOLOGY. - ISSN 1436-2228. - 17:2(2015), pp. 144-152. [10.1007/s10126-014-9602-z]
Abstract:
A screening among bacterial strains isolated from water-brine interface of the deep hypersaline anoxic basins (DHABs) of the Eastern Mediterranean was carried out for the biocatalytical resolution of racemic propyl ester of anti-2-oxotricyclo[2.2.1.0]heptan-7-carboxylic acid (R,S)-1, a key intermediate for the synthesis of d-cloprostenol. Bacillus horneckiae 15A gave highly stereoselective reduction of (R,S)-1, whereas Halomonas aquamarina 9B enantioselectively hydrolysed (R,S)-1; in both cases, enantiomerically pure unreacted (R)-1 could be easily recovered and purified at molar conversion below 57–58 %, showing the potential of DHAB extremophile microbiome and marine-derived enzymes in stereoselective biocatalysis.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Biocatalysis; Deep-sea hypersaline anoxic basins (DHABs); Esterase; Ketoreductase; Marine enzymes; Prostaglandin; Stereoselective
Elenco autori:
V. De Vitis, B. Guidi, M.L. Contente, T. Granato, P. Conti, F. Molinari, E. Crotti, F. Mapelli, S. Borin, D. Daffonchio, D. Romano
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