Antimicrobial resistance and class 1 and 2 integrons in Escherichia coli from meat turkeys in Northern Italy
Articolo
Data di Pubblicazione:
2014
Citazione:
Antimicrobial resistance and class 1 and 2 integrons in Escherichia coli from meat turkeys in Northern Italy / A. Piccirillo, D. Giovanardi, G. Dotto, G. Grilli, C. Montesissa, C. Boldrin, C. Salata, M. Giacomelli. - In: AVIAN PATHOLOGY. - ISSN 1465-3338. - 43:5(2014), pp. 396-405.
Abstract:
This study is aimed at determining the antimicrobial resistance (AMR) and the presence of class 1 and 2 integrons
in 48 avian pathogenic Escherichia coli (APEC) strains isolated from meat turkeys during three sequential
production cycles. Thirty avian faecal E. coli (AFEC) strains from the first cycle were also analysed. Strains were
tested for AMR against 25 antimicrobials by disk diffusion test and were screened for the presence of integrons
and associated gene cassettes by polymerase chain reaction followed by sequencing. Genetic relatedness of isolates
was established by pulsed-field gel electrophoresis. High levels of resistance were detected to tetracyclines,
penicillins and sulphonamides in APEC and AFEC. Resistance to aminoglycosides, fluoroquinolones,
cephalosporins and phenicols was variable, based on the antimicrobial drug and the isolate (APEC vs. AFEC).
Full susceptibility to colistin was detected. Multidrug resistance of up to seven antimicrobial classes was exhibited
by APEC (93.8%) and AFEC (100%). Nearly 44% of strains tested positive for class 1 and/or class 2 integrons
containing the dfrA, aadA and sat2 genes, alone or in combination, coding for streptomycin/spectinomycin,
trimethoprim and streptothricin resistance, respectively. The estX and orfF genes of unknown function were also
detected. A significant association was found between the presence of integrons and the resistance to
aminoglycosides and potentiated sulphonamides. The results of this study showed that AMR, multidrug resistance
and class 1 and 2 integrons are widespread among pathogenic and commensal E. coli from Italian turkeys. More
attention should be addressed to limit the use of antimicrobials in turkeys and the AMR of turkey E. coli.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
antimicrobial resistance ; turkeys ; class 1 and 2 integrons ; Escherichia coli
Elenco autori:
A. Piccirillo, D. Giovanardi, G. Dotto, G. Grilli, C. Montesissa, C. Boldrin, C. Salata, M. Giacomelli
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