Use of a O-benzyl-(S)-serine containing eluent for the efficient ligand-exchange chromatography-based enantioseparation of constrained glutamate receptor ligands
Articolo
Data di Pubblicazione:
2015
Citazione:
Use of a O-benzyl-(S)-serine containing eluent for the efficient ligand-exchange chromatography-based enantioseparation of constrained glutamate receptor ligands / F. Ianni, R. Sardella, A. Lisanti, N. Giacchè, P. Conti, A. Pinto, L. Tamborini, B. Natalini. - In: ANALYTICAL LETTERS. - ISSN 0003-2719. - 48:3(2015 Feb 11), pp. 383-395. [10.1080/00032719.2014.946040]
Abstract:
Four dihydroisoxazole prolines and four dihydroisoxazole cyclopentane derivatives were submitted to chiral ligand-exchange chromatographic analysis in the presence of O-benzyl-(S)-serine, as the chiral mobile phase additive to the eluent. The 1.0mM O-benzyl-(S)-serine and 0.5mM Cu(NO3)(2) eluent flowed at 1.0mL/min through a conventional octadecylsilica-based stationary phase maintained at 25 degrees C and provided excellent levels of enantioselectivity and resolution for all the species. By using enantiomers as model compounds, the method was validated revealing that the mixed ternary diastereomeric eluates present slightly different spectroscopic properties. The selected chiral ligand-exchange chromatography method was applied for semi-preparative enantioisolation that allowed the establishment of the k(-)
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Chiral mobile phase additive; Dihydroisoxazole amino acid derivatives; O-benzyl-(s)-serine; Chiral ligand-exchange chromatography
Elenco autori:
F. Ianni, R. Sardella, A. Lisanti, N. Giacchè, P. Conti, A. Pinto, L. Tamborini, B. Natalini
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