Quantitative trait loci mapping for conjugated linoleic acid, vaccenic acid and Δ9-desaturase in Italian Brown Swiss dairy cattle using selective DNA pooling
Articolo
Data di Pubblicazione:
2014
Citazione:
Quantitative trait loci mapping for conjugated linoleic acid, vaccenic acid and Δ9-desaturase in Italian Brown Swiss dairy cattle using selective DNA pooling / M.G. Strillacci, E. Frigo, F. Canavesi, Y. Ungar, F. Schiavini, L. Zaniboni, L. Reghenzani, M.C. Cozzi, A.B. Samorè, Y. Kashi, E. Shimoni, R. Tal Stein, M. Soller, E. Lipkin, A. Bagnato. - In: ANIMAL GENETICS. - ISSN 0268-9146. - 45:4(2014 Aug), pp. 485-499. [10.1111/age.12174]
Abstract:
A selective DNA pooling approach was applied to identify QTL for conjugated linoleic acid
(CLA), vaccenic acid (VA) and D9-desaturase (D9D) milk content in Italian Brown Swiss
dairy cattle. Milk samples from 60 animals with higher values (after correction for
environmental factors) and 60 animals with lower values for each of these traits from each
of five half-sib families were pooled separately. The pools were genotyped using the Illumina
BovineSNP50 BeadChip. Sire allele frequencies were compared between high and low tails
at the sire and marker level for SNPs for which the sires were heterozygous. An R procedure
was implemented to perform data analysis in a selective DNA pooling design. A correction
for multiple tests was applied using the proportion of false positives among all test results.
BTA 19 showed the largest number of markers in association with CLA. Associations
between SNPs and the VA and D9-desaturase traits were found on several chromosomes. A
bioinformatics survey identified genes with an important role in pathways for milk fat and
fatty acids metabolism within 1 Mb of SNP markers associated with fatty acids contents.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Bos taurus; genetic variability; milk fatty acids; pooled DNA
Elenco autori:
M.G. Strillacci, E. Frigo, F. Canavesi, Y. Ungar, F. Schiavini, L. Zaniboni, L. Reghenzani, M.C. Cozzi, A.B. Samorè, Y. Kashi, E. Shimoni, R. Tal Stein, M. Soller, E. Lipkin, A. Bagnato
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