Volatile fingerprint of Italian populations of Orchids using solid phase microextraction and gas chromatography coupled with mass spectrometry
Articolo
Data di Pubblicazione:
2014
Citazione:
Volatile fingerprint of Italian populations of Orchids using solid phase microextraction and gas chromatography coupled with mass spectrometry / A. Manzo, S. Panseri, I. Vagge, A. Giorgi. - In: MOLECULES. - ISSN 1420-3049. - 19:6(2014 Jun 06), pp. 7913-7936. [10.3390/molecules19067913]
Abstract:
The volatile fraction of Ophrys sphegodes Mill. subsp. sphegodes, Ophrys
bertolonii subsp. benacensis (Reisigl) O. Danesch, E. Danasch & Ehrend. and Neotinea tridentata (Scop.) R.M. Bateman, Pridgeon & M.W. Case, three orchid species with different pollinator attraction strategies, sampled in vivo and in situ, were evaluated by headspace solid phase microextraction coupled with gas-chromatography and mass
spectrometry. The results were compared with the volatile compounds emitted by flowering plant samples picked from the same populations of orchid species. Hydrocarbons, aldehydes, alcohols and terpenes were the major constituents of “in vivo” orchid scents and some distinctive differences in volatile metabolite composition were observed between Ophrys and Neotinea species. Moreover, the odour bouquets of the picked flowering plant samples were different from the in vivo ones and in particular different proportions of the various terpenes and an increase of α-pinene were observed. In conclusion HS/SPME GCMS proved to be a suitable technique for analyzing and distinguishing the volatile fingerprint of different orchid species, sampled in vivo and in situ in a non-disruptive way, with potentially great advantages for ecophysiological studies of rare and endangered species.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Conservation; Floral scent; HS/SPME GCMS; Italy; Orchidaceae
Elenco autori:
A. Manzo, S. Panseri, I. Vagge, A. Giorgi
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