Characterization of tetA-like gene encoding for a major facilitator superfamily efflux pump in Streptococcus thermophilus
Articolo
Data di Pubblicazione:
2014
Citazione:
Characterization of tetA-like gene encoding for a major facilitator superfamily efflux pump in Streptococcus thermophilus / S. Arioli, S. Guglielmetti, S. Amalfitano, C. Viti, E. Marchi, F. Decorosi, L. Giovannetti, D. Mora. - In: FEMS MICROBIOLOGY LETTERS. - ISSN 0378-1097. - 355:1(2014 Jun), pp. 61-70. [10.1111/1574-6968.12449]
Abstract:
Efflux pumps are membrane proteins involved in the active extrusion of a wide range of structurally dissimilar substrates from cells. A multidrug efflux pump named TetA belonging to the major facilitator superfamily (MFS) of transporters was identified in the Streptococcus thermophilus DSM 20617(T) genome. The tetA-like gene was found in the genomes of a number of S. thermophilus strains sequenced to date and in Streptococcus macedonicus ACA-DC 198, suggesting a possible horizontal gene transfer event between these two Streptococcus species, which are both adapted to the milk environment. Flow cytometry (single-cell) analysis revealed bistable TetA activity in the S. thermophilus population, and tetA-like gene over-expression resulted in a reduced susceptibility to ethidium bromide, tetracycline, and other toxic compounds even when the efflux pump was over-expressed in a strain naturally lacking tetA-like gene.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Streptococcus thermophilus; MFS efflux pump; antibiotic resistance; lactic acid bacteria
Elenco autori:
S. Arioli, S. Guglielmetti, S. Amalfitano, C. Viti, E. Marchi, F. Decorosi, L. Giovannetti, D. Mora
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