The major histocompatibility complex class II Ea promoter requires TFIID binding to an initiator sequence
Articolo
Data di Pubblicazione:
1996
Citazione:
The major histocompatibility complex class II Ea promoter requires TFIID binding to an initiator sequence / M. Bellorini, J.C. Dantonel, J.B. Yoon, R.G. Roeder, L. Tora, R. Mantovani. - In: MOLECULAR AND CELLULAR BIOLOGY. - ISSN 0270-7306. - 16:2(1996), pp. 503-512.
Abstract:
The major histocompatibility complex (MBC) class II Ea promoter is dependent on the presence of conserved upstream X and Y boxes and of initiator (Inr) sequences. In vitro transcription analysis of the Inr region with linker-scanning mutants pinpoints a functionally essential element that shows homology to the terminal deoxynucleotidyltransferase (TdT) Inr; contrary to the TdT Inr and other Inrs identified so far, the key sequence, between positions +5 and +12, is located within a transcribed area. Swapping the TdT sequence into the corresponding Ea position leads to a fivefold increase in transcription rate, without altering start site selection. Inr-binding proteins LBP-1/CP2 and TIP-a TdT Inr-binding protein unrelated to YY1-recognize the Ea Inr; they interact with overlapping yet distinct sequences around the Cap site, but their binding does not coincide with Ea Inr activity. A good correlation is, rather, found with binding of immunopurified holo-TFIID to this element. TFIID interacts both with Ea TATA-like and Inr sequences, but only the latter is functionally relevant. Unlike TBP, TMID binds in the absence of TFIIA, indicating a stabilizing role for TBP-associated factors in Ea promoter recognition. Sequence comparison with other mouse and human MHC class II promoters suggests a common mechanism of start site(s) selection for the MHC class II gene family.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Genes, MHC Class II ; Promoter Regions, Genetic ; Animals ; Base Sequence ; Binding Sites ; DNA Mutational Analysis ; DNA-Binding Proteins ; Humans ; Mice ; Molecular Sequence Data ; Phosphoproteins ; Point Mutation ; Protein Binding ; Transcription Factor TFIID; Transcription Factors; Transcription, Genetic ; Viral Proteins
Elenco autori:
M. Bellorini, J.C. Dantonel, J.B. Yoon, R.G. Roeder, L. Tora, R. Mantovani
Link alla scheda completa: