Data di Pubblicazione:
1995
Citazione:
CCAAT-box binding protein NF-Y (CBF, CP1) recognizes the minor groove and distorts DNA / A. Ronchi, M. Bellorini, N. Mongelli, R. Mantovani. - In: NUCLEIC ACIDS RESEARCH. - ISSN 0305-1048. - 23:22(1995 Nov 25), pp. 4565-4572. [10.1093/nar/23.22.4565]
Abstract:
The CCAAT box is one of the most common promoter elements. The evolutionarily conserved heteromeric factor NF-Y binds this sequence with high affinity and specificity. By comparing the methylation interference patterns of different sites, performing electrophoretic mobility shift assays (EMSA) with IC-substituted oligonucleotides and competition experiments with the minor groove binding (MGB) drugs distamicin A, tallimustine and Hoechst 33258 we show that NF-Y makes key minor groove interactions. Circular permutation assays on four CCAAT boxes, MHC Class II Ea, HSP70, ε-globin and MSV, indicate that NF-Y is able to distort the double helix by angles of 62-82°, depending on the site used, and suggest that nucleotides flanking the CCAAT pentanucleotide influence the degree of bending.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Nucleic Acid Conformation ; Promoter Regions, Genetic ; Base Sequence ; Binding Sites ; Biological Evolution ; CCAAT-Enhancer-Binding Proteins ; Conserved Sequence ; DNA ; DNA-Binding Proteins ; Methylation ; Molecular Sequence Data ; Oligodeoxyribonucleotides ; Plasmids ; Proteins ; Restriction Mapping ; Transcription Factors
Elenco autori:
A. Ronchi, M. Bellorini, N. Mongelli, R. Mantovani
Link alla scheda completa:
Link al Full Text: