Data di Pubblicazione:
2014
Citazione:
A Unique Mutation in a MYB Gene Cosegregates with the Nectarine Phenotype in Peach / E. Vendramin, G. Pea, L. Dondini, I. Pacheco, M.T. Dettori, L. Gazza, S. Scalabrin, F. Strozzi, S. Tartarini, D. Bassi, I. Verde, L. Rossini. - In: PLOS ONE. - ISSN 1932-6203. - 9:3(2014 Mar 03), pp. e90574.1-e90574.13. [10.1371/journal.pone.0090574]
Abstract:
Nectarines play a key role in peach industry; the fuzzless skin has implications for consumer acceptance. The peach/nectarine (G/g) trait was described as monogenic and previously mapped on chromosome 5. Here, the position of the G locus was delimited within a 1.1 cM interval (635 kb) based on linkage analysis of an F2 progeny from the cross ‘Contender’ (C, peach) x ‘Ambra’ (A, nectarine). Careful inspection of the genes annotated in the corresponding genomic sequence (Peach v1.0), coupled with variant discovery, led to the identification of MYB gene PpeMYB25 as a candidate for trichome formation on fruit skin. Analysis of genomic re-sequencing data from five peach/nectarine accessions pointed to the insertion of a LTR retroelement in exon 3 of the PpeMYB25 gene as the cause of the recessive glabrous phenotype. A functional marker (indelG) developed on the LTR insertion cosegregated with the trait in the CxA F2 progeny and was validated on a broad panel of genotypes, including all known putative donors of the nectarine trait. This marker was shown to efficiently discriminate between peach and nectarine plants, indicating that a unique mutational event gave rise to the nectarine trait and providing a useful diagnostic tool for early seedling selection in peach breeding programs.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
E. Vendramin, G. Pea, L. Dondini, I. Pacheco, M.T. Dettori, L. Gazza, S. Scalabrin, F. Strozzi, S. Tartarini, D. Bassi, I. Verde, L. Rossini
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