High prevalence of oqxAB in Escherichia coli Isolates from domestic and wild lagomorphs in Italy
Articolo
Data di Pubblicazione:
2014
Citazione:
High prevalence of oqxAB in Escherichia coli
Isolates from domestic and wild lagomorphs in Italy / G. Dotto, M. Giacomelli, G. Grilli, V. Ferrazzi, A. Carattoli, D. Fortini, A. Piccirillo. - In: MICROBIAL DRUG RESISTANCE. - ISSN 1076-6294. - 20:2(2014), pp. 118-123. [Epub ahead of print] [10.1089/mdr.2013.0141]
Abstract:
This study aimed to identify and characterize class 1 and 2 integrons and plasmid-mediated quinolones resistance
(PMQR) genes in a collection of 113 multidrug resistance (MDR) Escherichia coli isolated from farm and
wild lagomorphs between 2006 and 2008 in Northern Italy. Strains were examined for antimicrobial susceptibility
by agar disk diffusion method and E-test for colistin (COL); integrons and gene cassettes content by realtime
polymerase chain reaction (PCR) and DNA sequencing; PMQR genes by PCR and DNA sequencing; clonal
relatedness by multilocus sequence typing; and plasmids by PCR-based replicon typing. Class 1 integrons were
detected in 69 isolates (47 farm rabbits, 14 wild rabbits, and 8 wild hares). No class 2 integrons were found. Five
different gene cassettes arrays were identified (aadA1, dfrA1-aadA1, orf in682-dhfrA5, orf in682-dfrA5-orfD ins21,
and dfrA17-aadA5). Fifteen percent (17/113) of isolates carried oqxAB, no other PMQR determinants. All but one
oqxAB-positive E. coli strains were recovered from farm rabbits. Seven out of 17 strains were associated with the
predominant ST238 and carried from three to six different plasmid types, such as IncF, IncHI1, IncI1, IncN, IncP,
IncX1, IncY, and ColE. COL resistance was identified in 6/113 strains (5.3%). This study provides new insights
on the resistance phenotypes and the prevalence and dissemination of oqxAB in E. coli from farm and wild
lagomorphs, suggesting that these animals may be reservoir of these genetic determinants in Italy and thus a
potential source of PMQR E. coli for humans. PMQR mediated by oqxAB has not been detected in farm and wild
lagomorphs before.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Escherichia coli ; multidrug resistance (MDR) ; oqxAB ; lagomorphs
Elenco autori:
G. Dotto, M. Giacomelli, G. Grilli, V. Ferrazzi, A. Carattoli, D. Fortini, A. Piccirillo
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